Biological fluorescence detection
The real-time fluorescence quantitative pcrs are composed of a fluorescence quantitative system and a CCD system. Through the real-time monitoring of the CCD, the fluorescence of the cycle process is monitored. Computers connected to real-time devices collect fluorescence data. Data are displayed in chart form through the development of real-time analysis software. The raw data is drawn as a chart of fluorescence intensity relative to the number of cycles. After the original data is collected, analysis can begin. The software of the real-time device can normalize the collected data to make up for the difference in background fluorescence. After normalization, the domain value level can be set and the fluorescence data can be analyzed.
Real-time fluorescence quantitative pcr-meter light is widely used in basic medical research, pathogen detection, molecular cloning, genetic screening, gene expression, genotyping, genetic modification detection, food safety detection and public health epidemic monitoring and other industries and fields.